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Publication
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| Title of Article |
Proteomics detection of S100A6 in
tumor tissue interstitial fluid and
evaluation of its potential as a
biomarker of cholangiocarcinoma |
| Date of Acceptance |
26 February 2018 |
| Journal |
| Title of Journal |
Tumor Biology |
| Standard |
ISI |
| Institute of Journal |
SAGE |
| ISBN/ISSN |
10104283 |
| Volume |
|
| Issue |
40 |
| Month |
April |
| Year of Publication |
2018 |
| Page |
1-9 |
| Abstract |
Tumor interstitial fluid contains tumor-specific proteins that may be useful biomarkers for cancers. In this study, we identified
proteins present in cholangiocarcinoma interstitial fluid. Proteins derived from three samples of tumor interstitial
fluid and paired samples of adjacent normal interstitial fluid from cholangiocarcinoma patients were subjected to twodimensional
liquid chromatography with tandem mass spectrometry. Candidate proteins were selected based on a
greater than twofold change in expression levels between tumor interstitial fluid and normal interstitial fluid.
Upregulation of six proteins in tumor interstitial fluid, including S100 calcium binding protein A6 (S100A6), S100 calcium
binding protein A9, aldo-keto reductase family 1 member C4, neuropilin-1, 14-3-3 zeta/delta, and triosephosphate isomerase
was assessed by western blot and immunohistochemistry. Their potential as markers was evaluated in human
cholangiocarcinoma tissue arrays, and in serum using enzyme-linked immunosorbent assay. Expression of S100A6 was
higher in tumor interstitial fluid than in normal interstitial fluid and showed the highest positive rate (98.96%) in cholangiocarcinoma
tissues. Serum levels of S100A6 did not differ between cholangitis and cholangiocarcinoma patients, but
were significantly higher than in healthy individuals (p\0.0001). In cholangiocarcinoma cases, S100A6 level was associated
with vascular invasion (p = 0.007) and could distinguish cholangiocarcinoma patients from healthy individuals as effectively
as the carbohydrate antigen 19-9. In addition, potential for drug treatment targeting S100A6 and other candidate
proteins was also demonstrated using STITCH analysis. In conclusion, proteomics analysis of tumor interstitial fluid could
be a new approach for biomarker discovery, and S100A6 is a potential risk marker for screening of cholangiocarcinoma. |
| Keyword |
Cholangiocarcinoma, tumor interstitial fluid, proteomics, S100 calcium binding protein A6, biomarker |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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