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Publication
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| Research Title |
Antihypertensive and antioxidative effects of whole grain essential oil in nitric oxide deficient hypertensive rats |
| Date of Distribution |
14 September 2018 |
| Conference |
| Title of the Conference |
Europhysiology 2018 |
| Organiser |
The Physiological Society, the Scandinavian Physiological Society, the Deutsche Physiologische Gesellschaft and the Federation of European Physiological Societies |
| Conference Place |
The QEII Centre, Broad Sanctuary, Westminster, London SW1P 3EE, United Kingdom |
| Province/State |
กรุงลอนดอน ประเทศ อังกฤษ |
| Conference Date |
14 September 2018 |
| To |
16 September 2018 |
| Proceeding Paper |
| Volume |
2018 |
| Issue |
1 |
| Page |
265 |
| Editors/edition/publisher |
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| Abstract |
Antihypertensive and antioxidative effects of whole grain essential oil in nitric oxide deficient hypertensive rats
Hypertension is one of the major risk factors for cardiovascular disease (CVD). Nitric oxide (NO) plays an important role in the regulation of cardiovascular function. Inhibition of NO synthesis results in the development of hypertension. Oxidative stress is associated with the pathogenesis of hypertension. Consumption of healthy diet that contains natural antioxidants appears to reduce blood pressure and may decrease risk for CVD. Whole grain essential oil (WEO) extracted from brown rice contains high levels of dietary antioxidants which may protect against diseases related to oxidative stress. Therefore, this study aimed to investigate the antihypertensive effect of WEO in NO deficient hypertensive rats. Hypertension was induced in male Sprague-Dawley rats (200-230 g, n=6/group) by receiving N𝝎-nitro-L-arginine methyl ester (L-NAME), a NO synthase inhibitor, at a dose of 50 mg/kg/day in drinking water for 3 weeks. Simultaneously, L-NAME-treated rats were intragastrically administered daily with deionized water (DI) for an untreated control group or WEO (1 or 2 ml/kg) for treated groups. Rats received tap water and intragastrically administered with DI were served as normotensive controls. At the end of the experiment, rats were anaesthetized with an intraperitoneal injection of pentobarbital sodium (60 mg/kg). The left femoral artery and femoral vein were cannulated for continuously monitoring arterial blood pressure and for infusion of vasoactive agents, respectively. Rats were sacrificed by an overdose of the anaesthetic drug. Blood samples and thoracic aorta were collected for assay of oxidative stress markers. Data were expressed as means ± S.E.M., compared by ANOVA. Results showed that WEO significantly decreased arterial blood pressure of L-NAME-treated rats compared to normotensive controls (138.9 ± 5.8, 118.1 ± 3.7 vs. 160.4 ± 3.6 mmHg L-NAME control p<0.05). The vascular response to vasoconstrictor, phenylephrine (0.1 µM) was augmented while the response to endothelial-dependent vasodilator, acetylcholine (30 nM), was blunted in L-NAME-treated rats. WEO significantly improved the vascular responsiveness of L-NAME hypertensive rats (p<0.05). The antihypertensive effect of WEO was associated with a reduction in vascular superoxide production, plasma malondialdehyde and protein carbonyl (p<0.05). Moreover, WEO also increased eNOS expression and suppressed p47phox NADPH oxidase in the vascular tissues (p<0.05). The present results provide evidence for the antihypertensive and antioxidative properties of WEO and suggest that WEO might be useful as a dietary supplement against hypertension.
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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