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Publication
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| Research Title |
SUPPRESSION OF APOPTOSIS BY YANANG (Tilliacora triandra) IN METHOMYL STIMULATED RAW 264.7 MACROPHAGE |
| Date of Distribution |
1 November 2018 |
| Conference |
| Title of the Conference |
The 44th Congress on Science and Technology of Thailand (STT 44) |
| Organiser |
สมาคมวิทยาศาสตร์แห่งประเทศไทยในพระบรมราชูปถัมภ์ |
| Conference Place |
Bankok International Trad & Exhibition Center (BITEC), Bankok, Thailand |
| Province/State |
Bankok, Thailand |
| Conference Date |
29 October 2018 |
| To |
31 October 2018 |
| Proceeding Paper |
| Volume |
2018 |
| Issue |
1 |
| Page |
112 |
| Editors/edition/publisher |
- |
| Abstract |
Abstract:
Methomyl or S-methyl N-(methylcarmoyloxy) thioacetimidate is toxic chemical compound that widely used in agriculture. It belongs to the carbamate group, which has been well known in generating reactive oxygen species (ROS) and eventually leading to multi-organ toxicity. In addition, Methomyl has been reported as immunotoxic compound. Therefore, this research aimed to evaluate the detoxification efficiency of Yanang water extract (YWE) toward macrophage (RAW 264.7) cells stimulated by methomyl. At first, the cytotoxicity on Peripheral Blood Mononuclear Cells (PBMCs) and RAW 264.7 cells of YWE was evaluate by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The results obtained were found 2.5-10 μg/mL YWE had no effect on the viability of both PBMCs and RAW 264.7 cells. On the contrary, the cytotoxicity effect induced by 11,000 μM methomyl to RAW 264.7 cells was observed as evidence by significantly reduction of cell growth. A pronounced protective effect against this damage was observed upon co-incubation of the cells with 2.5, 5, and 10 µg/ml of YWE. The collected data from dual acridine orange/ethidium bromide fluorescent staining clearly demonstrate that methomyl induces apoptosis in RAW 264.7 cells, which was evidently decreased by co-treatment with YWE. In conclusion, our research suggested that Yanang extract might contain natural bioactive compounds which play an important role in detoxification of methomyl.
We would like to thank the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University for their financial assistance.
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย