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Publication
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| Research Title |
Effects of Oleic Acid and Palmitic Acid
on the Expression of Lipid-Biotransformation-Related Genes
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| Date of Distribution |
17 March 2018 |
| Conference |
| Title of the Conference |
The 10th Annual Northeast Pharmacy Research Conference of 2018 |
| Organiser |
Khon Kaen University |
| Conference Place |
Faculty of Pharmaceutical Science |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
17 March 2018 |
| To |
18 March 2018 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
157 |
| Editors/edition/publisher |
- |
| Abstract |
Introduction: Oleic acid (OA) and palmitic acid (PA) are saturated and unsaturated fatty acid, respectively, that mostly found in daily dietary especially western fried food. CYP4A11 is one of cytochrome P450s mainly responsible for ωω-1 hydroxylation in fatty acid metabolism.Some evidences have suggested that human CYP4A11 expression is related to peroxisome proliferator activated receptor alpha (PPAR-α). Moreover, OATP1B1, a well-known transporter, commonly expresses in the basolateral membrane of hepatocytes. However, regulatory pathway
of fatty acid is unclear. Human hepatocellular carcinoma cell (HepG2), human choriocarcinoma cell (BeWo), and human colorectal adenocarcinoma cell (Caco-2) are extensively used as models for study of xenobiotics/nutrients-metabolism. Hence, the effects of OA and PA on these lipid-biotransformation-related genes, namely, OATP1B1, CYP4A11, and PPAR-α, were determined in three cell lines. Materials and methods: OA or PA at the final concentration of 1 mM (in 0.5%isopropanol) was incubated with HepG2, BeWo, and CaCo2 cells (5x10 5
cells/mL in 6-well plate) for 24 h. Then, total RNA was isolated using acid guanidinium thiocyanate-phenol-chloroform method. Expressions of OATP1B1, CYP4A11, and PPAR-α mRNA were determined using RT-qPCR. Results: The expression of OATP1B1 mRNA was low abundant in BeWo and unchanged by either OA or PA. OA markedly induced the expression of OATP1B1 in HepG2 and CaCo2 while PA did not. The expression of CYP4A11 mRNA was higher abundant in HepG2 than BeWo. Interestingly, both OA and PA significantly induced the level of CYP4A11 mRNA in BeWo but not in HepG2. PPAR-α was not modified
by either OA or PA in HepG2 whilst PA extensively induced PPAR-α in BeWo. Conclusion: OA and PA might have different biotransformation. Moreover, organ-represented cell lines demonstrated different regulatory pathways of lipid metabolism. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย