| Abstract |
Biofilms are complex bacterial communities
have a mechanism for antibiotic resistance leading to
human health problems. It remains challenging to treat and
eradicate biofilms. In this work, the use of d-maltose synthesized
silver nanoparticles (AgNPs) was investigated in
an effort to eradicate a biofilm. AgNPs were synthesized
using a modified Tollen’s method. d-maltose was used
in synthesizing AgNPs with different concentrations of
d-maltose (0.01, 0.05 and 0.1 M), referred to as NP1, NP2
and NP3, respectively. TEM images revealed that the particles
were polygon shaped. The particle sizes were found
to be 86.81 ± 13.39, 54.94 ± 11.63 and 31.43 ± 31.76 nmdepending on their sugar concentrations. UV–Vis, ATR–
FTIR, and XRD patterns were employed to characterize the
AgNPs. Then, these AgNPs were investigated for their antibacterial
effects against Escherichia coli and Staphylococcus
aureus. Evaluation of the minimum inhibitory concentration
and minimal bactericidal concentration revealed that
S. aureus was inhibited by all AgNPs and killed by NP1
and NP3, and E. coli was inhibited and killed at all AgNPs
doses. Furthermore, anti-biofilm activity against these two
bacteria was observed using SEM and confocal laser scanning
microscopy. This sugar coated AgNPs is a promising
material for use in eradication of biofilms. |
Publication
Journal Publication
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