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Publication
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| Title of Article |
Physicochemical properties and oxygen affinity of
glutaraldehyde polymerized crocodile hemoglobin:
the new alternative hemoglobin source for
hemoglobin-based oxygen carriers |
| Date of Acceptance |
29 January 2019 |
| Journal |
| Title of Journal |
ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY |
| Standard |
SCOPUS |
| Institute of Journal |
Taylor and Francis |
| ISBN/ISSN |
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| Volume |
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| Issue |
47 |
| Month |
Mar |
| Year of Publication |
2019 |
| Page |
852-861 |
| Abstract |
Hemoglobin-based oxygen carriers (HBOCs) are modified stroma-free hemoglobin molecules used in
developing a blood substitute for therapeutic usage. In order to prevent hemoglobin dissociation, glutaraldehyde
(GTA) was used to generate high-molecular weight heterogeneous crocodile hemoglobin
(Poly-cHb). This work, Poly-cHb was created using various GTA concentrations, ranging from 0.025-
0.150% (v/v). Physicochemical properties were investigated that were comparable GTA polymerized
human hemoglobin (Poly-hHb). This study has revealed that GTA polymerization increases the molecular
size of Native-cHbs from 14.10nm over a range from 16.31 to 54.27 nm. Moreover, this polymerization
alters the secondary structure and heme environment by decreasing the helicity ratio from 1.00 to
0.95 at the highest condition and exhibits hypochromic shift of the Soret band to be 0.88 times lower
than the native. However, all Poly-cHbs still possessed higher oxygen affinity than that of Poly-hHbs
with average P50 values of 13 and 21mmHg, respectively. Although, polymerization affected the overall
Poly-cHb structure slightly, but compensated by decreasing the denaturation level to lower than
10%. Thus, it is interesting to note that Poly-cHb may advantageously provide effective oxygen carriage
and ability for pasteurization, which may benefit the search for new alternative hemoglobin sources for
HBOC development. |
| Keyword |
Hemoglobin-based oxygen carrier (HBOC); polymerization; modified hemoglobin; stroma-free hemoglobin; Siamese crocodile; glutaraldehyde |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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