| Abstract |
Plant-derived active compounds are exists in medicinal plants and herbs; they consist of various kinds of phytochemical molecules known to have oxidative scavenger capacity in addition to other biological activates. In this study four different plants (Panax ginseng, Alpinia galanga, Vitis vinifera and Moringa oleifera) and plant parts (rhizomes, leaves and seeds) were used to investigate neuroblastoma cytotoxicity and determine their neuroprotective activity against H2O2 stress. Plant parts were dried, powdered and extracted with 95% ethanol for Alpinia galanga and with 70% ethanol for Panax ginseng, Vitis vinifera and Moringa oleifera then were evaporated by rotary evaporator. Cytotoxicity was determined for each extract by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT assay). IC40 of each extract was chosen to test neuroprotective role against H2O2 radicals. SHSY5Y neuroblastoma cells were incubated with extracts individually as triplicate for 6 hrs and then exposed to 50 µM H2O2 for 1 hr. The protected viable SHSY5Y neuroblastoma cells were investigated by MTT assay. results showed that Panax ginseng rhizomes extract gave highest neuroprotective activity followed by Alpinia galanga leaves extract as 51% and 44% viable cell, respectively. Neuroprotection effect by Alpinia galanga activity rhizomes was 40% while Vitis vinifera, Panax ginseng leaves and Moringa oleifera leaves extracts showed law protection activity against H2O2. It concluded that Panax ginseng rhizomes extract exhibited the highest neuroprotection effect among others. |
Publication
Journal Publication
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