| Title of Article |
Roles of Zinc Finger Protein 423 in Proliferation and
Invasion of Cholangiocarcinoma through
Oxidative Stress |
| Date of Acceptance |
3 July 2019 |
| Journal |
| Title of Journal |
Biomolecules |
| Standard |
ISI |
| Institute of Journal |
Multidisciplinary Digital Publishing Institute (MDPI) |
| ISBN/ISSN |
2218-273X |
| Volume |
2019 |
| Issue |
7 |
| Month |
|
| Year of Publication |
2019 |
| Page |
doi.org/10.3390/biom9070263 |
| Abstract |
Zinc finger protein 423 (ZNF423) is a transcriptional factor involved in the development
and progression of cancers but has not yet been examined in cholangiocarcinoma (CCA), an oxidative
stress-driven cancer of biliary epithelium. In this study, we hypothesized that oxidative stress
mediated ZNF423 expression regulates its downstream genes resulting in CCA genesis. ZNF423
protein expression patterns and 8-oxodG (an oxidative stress marker) formation in CCA tissues
were investigated using immunohistochemical analysis. The results showed that ZNF423 was
overexpressed in CCA cells compared to normal bile duct cells adjacent of the tumor. Notably,
ZNF423 expression was positively correlated with 8-oxodG formation. Moreover, ZNF423 expression
in an immortalized cholangiocyte cell line (MMNK1) was increased by hydrogen peroxide-treatment,
suggesting that oxidative stress induces ZNF423 expression. To investigate the roles of ZNF423 in
CCA progression, ZNF423 mRNA was silenced using specific siRNA in CCA cell lines, KKU-100
and KKU-213. Silencing of ZNF423 significantly inhibits cell proliferation and invasion of both CCA
cell lines. Taking all these results together, the present study denoted that ZNF423 is an oxidative
stress-responsive gene with an oncogenic property contributing to the regulation of CCA genesis. |
| Keyword |
zinc finger protein 423 (ZNF423); oxidative stress; cholangiocarcinoma; cancer progression |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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