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Publication
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| Research Title |
Singlet oxygen formation in photodynamic therapy using guaiazulene and red laser: an in vitro study |
| Date of Distribution |
30 June 2019 |
| Conference |
| Title of the Conference |
17th International Photodynamic Association World Congress |
| Organiser |
SPIE |
| Conference Place |
Boston Marriott Cambridge |
| Province/State |
Cambridge, Massachusetts, USA |
| Conference Date |
28 June 2019 |
| To |
4 July 2019 |
| Proceeding Paper |
| Volume |
11070 |
| Issue |
- |
| Page |
110709J |
| Editors/edition/publisher |
Tayyaba Hasan |
| Abstract |
Guaiazulene is one of the natural azulene derivatives, which has anti-inflammatory as well as photosensitizer property and can be activated by highly penetrating red light. The proper singlet oxygen from this activation will lead to a useful strategy for specific treating unwanted cells.
Objective
To measure in vitro amount of singlet oxygen produced from PDT reaction using various guaiazulene concentrations and red laser energies.
Methods
Guaiazulene were prepared by diluting in methanol and mixed with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide (TPC) to obtain 1, 2, 5, 15, 25, 35, 100, and 500 µM. Erythrosine (10 µM in each light energies) were used as positive control. Then, irradiations were performed with various red laser energies (625 nm, 151.5 mW/cm2, distance from laser tip to bottom of the well = 8 mm, and continuous mode) at 0, 4, and 8 J/cm2 in a black 96-well plate. The amount of singlet oxygen was measured in triplicate by electron spin resonance spectrometer (Bruker, Germany) at 0, 3, and 5 minutes after irradiation. Kruskal-Wallis and Mann-Whitney U test were used for statistical analyses with significant differences at p-value < 0.05.
Results
The average singlet oxygen of 5 µM group significantly different from no guiazulene group (p < 0.01). Regarding the energy, both 4 and 8 J/cm2 resulted in relatively equal amounts of singlet oxygen.
Conclusion
Five µM of guaiazulene activated by 625 nm laser at energy densities 4 - 8 J/cm2 dramatically generated singlet oxygen in current in vitro setting.
Keyword Photodynamic therapy, Singlet oxygen, Guaiazulene, Red laser, Electron spin resonance spectrometer, TPC
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
11
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Journal Publication
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