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Publication
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| Title of Article |
Expression of endogenous phospholipase D1, localized in mouse submandibular gland, is greater in females and suppressed by testosterone |
| Date of Acceptance |
19 July 2019 |
| Journal |
| Title of Journal |
Journal of Anatomy |
| Standard |
SCOPUS |
| Institute of Journal |
anatomical society of great britain and ireland |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2019 |
| Page |
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| Abstract |
In order to clarify the signal transduction mechanism in the differentiation and secretion of salivary glandular cells, the present study was attempted to examine in the submandibular gland (SMG) of mice, the expression and localization of phospholipase D1 (PLD1), one of important effector molecules working in response to the activation of intramembranous receptors by first messengers. In immunoblotting analysis, the expression of PLD1 was high at postnatal 4 week (P4W) and decreased at P8W, while it was at negligible levels at newborn stage (P0W) and postnatal 2 week (P2W). The expression of PLD1 was greater in females, and it was suppressed by administration of testosterone administration in to female mice. In immuno-light microscopy, immunoreactivity for PLD1 at P4W was moderate to intense in forms of dots and globules mainly in the apical domains of immature granular convoluted tubule (GCT)-cells localized largely in the proximal portion of the female GCT. By P8W, it decreased in intensity and remained weak to moderate along the apical plasmalemma of cells throughout the course of the female GCT, whereas it was faint throughout the GCT of the male SMG at P4W and negligible at P8W. In immuno-electron microscopy, immature GCT-cells characterized by electron-lucent granules were immunoreactive and the immunoreactive materials were deposited close to, but not within those granules. Typical GCT-cells, characterized by electron-dense granules, were immunonegative. No significant immunoreaction for PLD1 was seen in acini of SMGs of either sex at any time points examined. It is suggested that PLD1 is involved in the signaling for secretion of immature GCT cells and influences differentiation of these cells probably through their own secretory substances. |
| Keyword |
PLD1, Expression, Localization, Female dominance, Testosterone suppression, Immature granular convoluted tubule |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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