| Research Title |
Effect of vancomycin on branched-chain and straight-chain fatty acids of vancomycin-susceptible and non-susceptible Staphylococcus aureus
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| Date of Distribution |
12 November 2019 |
| Conference |
| Title of the Conference |
International Conference of the 40th Anniversary of AMS-KKU Foundation in Conjunction with the 4th Allied Health Sciences Symposium |
| Organiser |
The Faculty of Associated Medical Sciences , Khon Kaen University, Khon Kaen, Thailand 40002. |
| Conference Place |
ANANI KHON KAEN HOTEL |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
6 November 2019 |
| To |
7 November 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
- |
| Page |
60 |
| Editors/edition/publisher |
- |
| Abstract |
Staphylococcus aureus membrane fatty acids are generally contained a mixture of branched-chain fatty acids (BCFAs) and straight-chain fatty acids (SCFAs) which the BCFA function is an increasing membrane fluidity and SCFAs decrease membrane fluidity. The objective of this study was to determine the cellular fatty acid methyl ester profile among clinical S. aureus isolates including vancomycin-susceptible S. aureus (VSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus (VISA).
Thirty S. aureus isolates were cultivated on media with and without vancomycin before conducted to extract and analyzed the cellular fatty acids by Gas chromatography with flame ionization technique.
This study showed that vancomycin had a significant impact on cellular fatty acids level of S. aureus. The membrane fluidity of VSSA cells had reduced a total level of SCFAs (p<0.05) and increased a total level of BCFAs under vancomycin stress (p<0.05). However, vancomycin can induce the change of only BCFAS but no effect on SCFAs in hVISA while vancomycin had no effect on both BCFAs and SCFAs in VISA cells. Therefore, we concluded that vancomycin has much impact on VSSA followed by hVISA, but no effect on VISA cell.
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย