| Title of Article |
Attenuated total reflection: Fourier transform infrared spectroscopy for detection of
heterogeneous vancomycin—intermediate
Staphylococcus aureus |
| Date of Acceptance |
17 December 2019 |
| Journal |
| Title of Journal |
World Journal of Microbiology and Biotechnology |
| Standard |
ISI |
| Institute of Journal |
© Springer Nature Switzerland AG. |
| ISBN/ISSN |
ISSN: 0959-3993 (Print) 1573-0972 (Online) |
| Volume |
2020 |
| Issue |
36 |
| Month |
January |
| Year of Publication |
2020 |
| Page |
1-12 |
| Abstract |
Staphylococcus aureus strains resistant to the last line antibiotic, vancomycin, have been of clinical concern. These include
heterogeneous vancomycin-intermediate S. aureus (hVISA) and VISA. The hVISA phenotype cannot be detected by routine
laboratory methods. Characterization of hVISA/VISA by new technologies is necessary to differentiate them rapidly from
the vancomycin-susceptible isolates (VSSA). In this study, we developed a model for discrimination of hVISA from VSSA
by using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with multivariate data
analysis, displaying a phenotypic signature of the bacteria. ATR-FTIR spectra were acquired from a total of 59 clinical
methicillin-resistant S. aureus (MRSA) isolates comprising 28 hVISA and 31 VSSA strains. Principal component analysis
(PCA) and partial least square discriminant analysis (PLS-DA) were used to analyze 351 spectra of 39 isolates and develop
a discrimination model for identifying hVISA and VSSA. The classification model, which was used for blind testing of 90
spectra from each of 10 hVISA, and 10 VSSA isolates, provided 100% sensitivity and specificity. The modeling revealed
that the major discrimination between hVISA and VSSA phenotypes involved bands related to cell wall content (1087 and
1057 cm−1). This study showed that ATR-FTIR technique may be an alternative method for rapid detection of low-level
vancomycin-resistant S. aureus. |
| Keyword |
Fourier transform infrared spectroscopy, Heteroresistance, Methicillin-resistant staphylococcus aureus, Partial least square discriminant analysis, Vancomycin resistance |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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