| Research Title |
Alternative method for isolating HDL with a small sample volume |
| Date of Distribution |
6 November 2019 |
| Conference |
| Title of the Conference |
International Conference of the 40th Anniversary of AMS-KKU Foundation in Conjunction with the 4th Allied Health Sciences Symposium |
| Organiser |
Faculty of Associated Medical Sciences, Khon Kaen University |
| Conference Place |
Avani Khon Kaen Hotel and Convention Center |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
6 November 2019 |
| To |
8 November 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
64 |
| Editors/edition/publisher |
AMS AHSS 2019 |
| Abstract |
A plethora of evidences have shown that HDL can be used as a biomarker in the diagnosis of several diseases. Various publications showed that isolating HDL requires a large sample volume. Thus, the limited sample volume sometimes becomes a problem in research and diagnostic needs. This study aimed to isolate HDL from serum samples with small volume variations of 200 and 500 µL. HDL was isolated using the HDL Purification Kit (Cell Biolabs) and continued with the measurement of protein concentration using the BCA protein assay. To ensure the success of HDL isolation, ApoA-1 was used as an HDL biomarker and detected using Western Blot. The results showed HDL isolated from 200 and 500 µL of serum had protein concentration of 7.125 and 9.375 µg / µL respectively and they showed the presence of ApoA-1 (~29 kDa). It can be concluded that this method can be used as an alternative method to isolate HDL with a small sample volume. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย