| Research Title |
Anti-proliferative and anti-migratory effects of β-sitosterol on oral squamous cell carcinoma cells |
| Date of Distribution |
8 July 2019 |
| Conference |
| Title of the Conference |
DFCT 2019 The 17th International Scientific Conference of the Dental Faculty Consortium of Thailand |
| Organiser |
the Dental Faculty Consortium of Thailand |
| Conference Place |
Pullman Khon Kaen Raja Orchid Khon Kaen, Thailand |
| Province/State |
ขอนแก่น |
| Conference Date |
8 July 2019 |
| To |
10 July 2019 |
| Proceeding Paper |
| Volume |
17 |
| Issue |
1 |
| Page |
331-337 |
| Editors/edition/publisher |
Assoc. Prof. Jarin Paphangkorakit |
| Abstract |
Background: β-sitosterol is a main dietary phytosterol
found in plants such as nuts and berries. The structure
of phytosterols is similar to cholesterol which is one of
the major components of cell membrane. β-sitosterol
has been shown to promote cell function, reduce blood
cholesterol, reduce risk of heart disease and prevent
cancer formation. The anti-cancer property of β-sitostero
has been demonstrated in many cancer cells including
stomach cancer cells, colon cancer cells and human
prostate cancer cells. However, there is no previous
study on its anti-cancer potential in oral cancer cells.
Objectives: The aim of this current study was to investigate the anti-proliferative and anti-migratory effects of β-sitosterol against oral squamous cell carcinoma cells.
Methods: The anti-proliferative effect of β-sitosterol
against two human oral cancer cell lines, ORL-48 and
ORL-136 was assessed by the MTT assay whereas the
anti-migratory effect was determined by the wound
healing assay.
Results: β-sitosterol induced a significant, dose-dependent, proliferation inhibition of ORL-48 and ORL-136 cells. IC50 of β-sitosterol for ORL-48 at 24 and 48 h is 7.22 μg/mL and 4.09 μg/mL, respectively while IC50 of β-sitosterol for ORL-136 at 24 and 48 h is 27.58 μg/mL and 3.77 μg/mL, respectively. In addition, β-sitosterol remarkably inhibited ORL-48 and ORL-136 cell migration demonstrated by the wound healing assay in a dosedependent manner.
Conclusion: In summary, these results suggest that
β-sitosterol can act as a potent inhibitor of proliferation
and migration of ORL-48 and ORL-136 oral cancer cells.
Further studies are required to investigate the underlying mechanism and its potential use as an anti-cancer agent against oral squamous cell carcinoma cells. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
10
|
|
|
Publication
Journal Publication
ไทย