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Publication
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| Title of Article |
Evaluating Sugarcane Genotypes for Genetic Variation with Differential Sucrose Accumulation Using TRAP Markers and Partial Sai Nucleotide Polymorphism |
| Date of Acceptance |
31 July 2018 |
| Journal |
| Title of Journal |
ScienceAsia |
| Standard |
SCOPUS |
| Institute of Journal |
Science Society of Thailand, Faculty of Science, Chulalongkorn University |
| ISBN/ISSN |
Evaluating sugarcane genotypes for genetic variation with differential sucrose accumulation using TRAP markers and partial Sai nucleotide polymorphism |
| Volume |
2019 |
| Issue |
45 |
| Month |
July |
| Year of Publication |
2020 |
| Page |
309-317 |
| Abstract |
This study was conducted to characterize 17 sugarcane genotypes from Hawaii and Thailand using 12
target region amplification polymorphism (TRAP) markers and partial Sai nucleotide polymorphism. A total of 275
fragments were produced, of which 273 (99.27%) were polymorphic. The polymorphic information content (PIC)
ranged from 0.912–0.959 with an average value of 0.938. Genetic similarity (GS) by Dice’s similarity coefficient ranged
from 0.19–0.81 with a mean of 0.44. The 14 sugarcane genotypes from the Thailand collection with different sugar
content were also assessed. A total of 198 fragments were found, of which 174 (87.88%) were polymorphic. The
PIC value ranged from 0.860–0.937 with an average value of 0.914. The GS ranged from 0.49–0.85 with a mean of
0.71. The dendrograms constructed by the UPGMA cluster indicate strong differentiation between the Thai sugarcane
genotypes and the Hawaiian sugarcane genotypes. In addition, S. officinarum is the group most closely associated with
modern sugarcane. The partial Sai nucleotide sequence of 16 sugarcane genotypes from Hawaii and Thailand comprised
608–692 bp, within exon 2. There were 49 single nucleotide polymorphisms (SNPs) and two sites of variation including
insertion and deletion (In/Del). Sai nucleotide polymorphism data matrices produced a more refined phylogenetic tree
that shows four distinct groups. The results derived from nucleotide sequencing were somewhat similar to those derived
from the TRAP markers. Thus, the utilization of informative TRAP and Sai nucleotide sequencing for a genetic variation
study can result in the selection of diverse sugarcane parents for trait of sucrose content. |
| Keyword |
Saccharum, phylogenetic tree, sucrose content, TRAP marker, soluble acid invertase |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย