| Abstract |
Photoaging effect of solar radiation can be prevented by application of broad spectrum sunscreens onto skin. Recently, the combinations of the synthetic sunscreens with natural antioxidants have gained an interest due to their abilities to protect the skin from ultraviolet ( induced reactive oxygen species generation. Grape seed extract (GSE) is a source of polyphenol antioxidants with anti inflammatory properties accumulating for anti aging capability. This study is aimed to access the effect of GSE on nitric oxide (NO) inhibition using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and its protective capacity against UV and visible light radiation. The anti inflammatory effect of GSE was determined by nitric oxide inhibition assay using gallic acid as the positive control. The UV visible absorption spectrum of GSE alone and in combination with UV filters, including octyl methoxycinnamate (OMC) and Avobenzone were examined in a range of 200 800 nm by UV spectrophotometer. The results showed that GSE at concentration 100 μg/ml significantly inhibited production of NO in LPS -stimulated RAW 264.7 cells demonstrating promising anti inflammatory properties. In addition, GSE showed the ability to absorb broad range of the solar spectrum including UV and blue light. Moreover, combinations of GSE with UV filters broaden the absorption spectra of both OMC and Avobenzone from UVB and UVA, respectively, to the visible spectrum of longer wavelengths. This study demonstrates promising results that could become the basis for development of sunscreen containing GSE with broad spectrum action. |
Publication
Journal Publication
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