|
|
Publication
|
| Title of Article |
RGB Colorimetric Method for the Quantitative Analysis of Levocetirizine Tablets |
| Date of Acceptance |
22 June 2020 |
| Journal |
| Title of Journal |
Isan Journal of Pharmaceutical Sciences |
| Standard |
TCI |
| Institute of Journal |
คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น มหาวิทยาลัยมหาสารคาม มหาวิทยาลัยอุบลราชธานี |
| ISBN/ISSN |
ISSN 1905-0852 |
| Volume |
16 |
| Issue |
3 |
| Month |
July-September |
| Year of Publication |
2020 |
| Page |
78-88 |
| Abstract |
This study presented a simple and not time-consuming method coupled with a RGB colorimetric technique using the
built-in function of mobile phone digital imaging devices for quantitative analysis. The method was based on the detection of
color intensities and the evaluation of relationship between measured color and concentration of sample. Materials and methods:
The proposed method was applied successfully to analyze levocetirizine (LCTZ) quantitatively in pharmaceutical tablet dosage
forms using bromocresol green (BCG) as a coloring agent. Bromocresol green solution only is a blue color. When it reacted with
levocetirizine it formed a yellow-green complex. The intensity of yellow-green complex color was depending on the concentration
of drug sample. The complex solutions were transferred into standard glass cuvettes and then the images were recorded by
mobile phone. The pixel array reader program was applied to evaluate the red-green-blue intensities (RGB scale) of the recorded
images that were converted to the concentration which was calculated from the relationship of linear equation. Under the
optimized condition, a calibration curve was established by the RGB scale and concentration of drug standard. Validation
procedure was achieved concerning linearity, accuracy, and precision of developing method. Results: The proposed method
obeyed Beer’s law and show linear relationship in concentration range of 2 - 40 µg mL-1 of levocetirizine with a good coefficient
of determination (r2 = 0.9996). The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.64 and 4.96 µg
mL-1, respectively. The common excipients used as additives in the pharmaceutical dosage form had shown no effect on the
proposed method. The results acquired by the proposed method were compared favorably with those acquired by the reference
method at a 95% confidence level with no significant difference (n=6). Conclusion: The developed method had shown accurate,
precise, and reproducible. Moreover, this demonstrates the possibility of RGB colorimetric technique in the quantitative analysis
of drugs in their pharmaceutical dosage forms with a simple procedure, low cost, and more applications for pharmaceutical
analysis are expected to be use as an alternative method. |
| Keyword |
Levocetirizine, Digital imaging, RGB intensities, Colorimetric method |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
ชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย