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Publication
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| Research Title |
Tyrosinase inhibitory activity of crocodile (Crocodylus siamensis) blood components and its active peptide |
| Date of Distribution |
11 July 2017 |
| Conference |
| Title of the Conference |
The 5th Conference of the Asia Pacific Protein Association with the 12th International Symposium of the Protein Society of Thailand |
| Organiser |
Protein Society of Thailand (PST) and the Chulabhorn Research Institute |
| Conference Place |
The Tide Resort |
| Province/State |
Bangsaen, Chonburi, Thailand |
| Conference Date |
11 July 2017 |
| To |
14 July 2017 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
141 |
| Editors/edition/publisher |
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| Abstract |
Melanin is an essential skin pigment which provides beneficial effects in preventing skin damage under normal conditions. However, the accumulation of abnormal melanin in the skin may induce a variety of hyperpigmentation disorders. The melanin synthesis process is conducted in melanocytes and is controlled by tyrosinase. Hence, many tyrosinase inhibitors have been numerous investigated with the purpose of developing skin whitening cosmetics and hyperpigmentation disorder treatments. Although, several tyrosinase inhibitors have been developed until to date, many therapeutics still suffer from adverse cytotoxic and mutagenic effects. As a result, it is necessary to find natural bioactive compounds that display anti-tyrosinase activity while showing relatively low incidences of detrimental side effects. Therefore, this study aimed to investigate the effect of potent protein or peptide constituents of the major components of crocodile blood (i.e. serum, plasma, white blood cell extract, hemoglobin and Leucrocin I peptide) on tyrosinase inhibitory activity. It was found that among all investigated blood components, white blood cell extract at 1.70 mg protein/ml showed the highest inhibitory activity of about 32.10 ± 1.57%. In a previous study, we sequenced and described a novel antimicrobial peptide termed Leucrocin I (NGVQPKY), which was purified from crocodile white blood cell extract. This peptide contained specific amino acid residues deemed essential for tyrosinase activity inhibition, such as phenylalanine and valine. Thus, Leucrocin I was synthesized and also assayed in terms of anti-tyrosinase activity. The collected data demonstrates that Leucrocin I at a concentration of 2 mM exhibited an inhibitory activity off 24.82 ± 2.40%, suggesting that Leucrocin I is one of the main tyrosinase inhibiting peptides contained in white blood cell extract. In conclusion, white blood cell extract and Leucrocin I may bear great potential in the development of depigmentation agent for the treatment of hyperpigmentation disorders and cosmetic products. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย