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Publication
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| Title of Article |
Reversine, a selective MPS1 inhibitor, induced autophagic cell death via diminished glucose uptake and ATP production in cholangiocarcinoma cells |
| Date of Acceptance |
2 December 2020 |
| Journal |
| Title of Journal |
PeerJ |
| Standard |
ISI |
| Institute of Journal |
PeerJ Inc. |
| ISBN/ISSN |
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| Volume |
2021 |
| Issue |
9 |
| Month |
January |
| Year of Publication |
2021 |
| Page |
e10637 |
| Abstract |
Reversine is a selective inhibitor of mitotic kinase monopolar spindle 1 (MPS1) and
has been reported as an anticancer agent in various cancers. The effects of reversine on
bile duct cancer, cholangiocarcinoma (CCA), a lethal cancer in Northeastern Thailand,
were investigated. This study reports that reversine inhibited cell proliferation of CCA
cell lines in dose- and time-dependent manners but had less inhibitory effect on an
immortalized cholangiocyte cell line. Reversine also triggered apoptotic cell death by
decreasing anti-apoptotic proteins, Bcl-XL and Mcl-1, increasing Bax pro-apoptotic
protein and activating caspase-3 activity. Moreover, reversine induced autophagic cell
death by increasing LC3-II and Beclin 1 while decreasing p62. Reversine activated
autophagy via the AKT signaling pathway. Additionally, this study demonstrated for the
first time that reversine could diminish the expression of Hypoxia-Inducible Factor 1-
alpha (HIF-1 ) and glucose transporter 1 (GLUT1), resulting in a reduction of glucose
uptake and energy production in CCA cell lines. These findings suggest that reversine
could be a good candidate as an alternative or supplementary drug for CCA treatment. |
| Keyword |
Reversine, MPS1, Cholangiocarcinoma, Autophagy, Apoptosis, GLUT1 |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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