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Publication
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| Research Title |
Tyrosinase inhibition and antioxidant activity of Leucrocin I and its derivatives |
| Date of Distribution |
20 June 2018 |
| Conference |
| Title of the Conference |
The 6th International Conference on Biochemistry and Molecular Biology (BMB 2018) |
| Organiser |
The Biochemistry and Molecular Section of the Science Society of Thailand and Department of Biochemistry, Faculty of science, Chulalongkorn University |
| Conference Place |
Rayong Resort |
| Province/State |
Rayong, Thailand |
| Conference Date |
20 June 2018 |
| To |
22 June 2018 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
37 |
| Editors/edition/publisher |
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| Abstract |
This research was aimed to investigate tyrosinase inhibitory activity and antioxidant activity of antimicrobial peptide, Leucrocin I. Our results demonstrated that it showed uncompetitive inhibitory property with IC50 of 401.97 ± 9.55 µM and 205.00 ± 10.32 µM toward monophenolase and diphenolase, respectively, but lacking antioxidant activity. To improve the tyrosinase inhibitory activity and antioxidant activity, Leucrocin I-based design peptide was performed. Three rationally designed peptides, T1, T2 and T3, were obtained with non-hemolysis activity was detected toward red blood cell. T1 and T2 exhibited competitive inhibition with IC50 toward diphenolase less than Leucrocin I approximately 1.48- and 1.60-fold, respectively. Furthermore, T1 and T2 dramatically increased lag time of monophenolase activity. In terms of T3, both diphenolase and monophenolase inhibitory activity were dramatically decreased when compared to Leucrocin I. CD spectroscopy indicated that Leucrocin I, T1 and T2 caused the change of native secondary structure of tyrosinase resulting in decreasing of tyrosinase activity. Moreover, T1 and T2 exhibited stronger antioxidant activity toward DPPH and ABTS radical than that of Leucrocin I. These results suggested that Leucrocin I, T1 and T2 are promising peptide to develop as ingredients contained in skin whitening cosmetic and hyperpigmentation treatment agent. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย