|
|
Publication
|
| Research Title |
The effect of traditional Thai massage investigated on NK cell subsets using flow cytometry analysis |
| Date of Distribution |
7 November 2019 |
| Conference |
| Title of the Conference |
International Conference of the 40th Anniversary of AMS-KKU Foundation in Conjunction with the 4th Allied Health Sciences Symposium “Research and Innovations in Transformative and Translational Health Sciences” |
| Organiser |
Faculty of Associated Medical Sciences, Khon Kaen Universuty |
| Conference Place |
Avani hotel Khon Kean |
| Province/State |
Khon Kaen |
| Conference Date |
6 November 2019 |
| To |
8 November 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
1 |
| Editors/edition/publisher |
|
| Abstract |
Introduction: Traditional Thai Massage (TTM) is an exclusive technique of deep tissue massage by acupressure along ten meridian lines or Sen sib in Thai and muscle stretching at the end. It has been known as an alternative treatment supposed that promote physical, mental relaxation, reduce myofascial pain syndrome, and improve immunity. Previous studies have shown that TTM affected to change the number of Natural Killer (NK) cell after being massaged immediately. However, the experimental design for measurement the changes involving TTM and NK cell subsets at different effect times using flow cytometer has few studied yet.
Methods: The study design was performed a randomized crossover trial of having 6 sessions of 1-hour TTM/rest (once weekly) with a 30-day washout period. 12 volunteers, aged 67.7±5.12 years were recruited from a community in the North-East of Thailand. Blood samples were collected on the baseline, the short-term effect for within 60 minutes after single interventions and the long-term effect for 5 weeks after either weekly interventions consecutively. The immunophenotyping was specifically investigated for cell surface markers using monoclonal antibody conjugated with different fluorochromes staining by BD FACSCanto II Flow cytometer.
Results: The data analysis were categorized NK cell subsets by NK cell (CD3-CD56+), NKG2D+ on NK cell (CD3-CD56+NKG2D+), NKG2D- on NK cell (CD3-CD56+NKG2D-), NKdim cell (CD3-CD56+dim), and NKbright cell (CD3-CD56+bright). The result showed that significant mean difference within effect time of TTM group on NK cell (p=0.039), NKdim cell (p=0.044), and NKbright cell (p=0.040).
Conclusions: Consequently, this study has exposed the appropriate study design and analytical method using flow cytometry to simulate the effect of TTM on NK cell subsets that may have significant immune-modulating effects.
|
| Author |
|
| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย