|
Publication
|
Research Title |
Serum Cytokine-induced apoptosis inhibitor 1 level as a diagnostic biomarker for cholangiocarcinoma |
Date of Distribution |
18 December 2020 |
Conference |
Title of the Conference |
The 1st CARI Annual Conference 2020 |
Organiser |
Cholangiocarcinoma research institute, Faculty of Medicine, KKU |
Conference Place |
LE CASSIA HOTEL |
Province/State |
Khon Kaen, Thailand |
Conference Date |
18 December 2020 |
To |
19 December 2020 |
Proceeding Paper |
Volume |
None |
Issue |
None |
Page |
None |
Editors/edition/publisher |
|
Abstract |
Introduction: The mortality rate of cholangiocarcinoma (CCA) is high since there is a lack of non-invasive approaches able to accurately detect this silent tumor at early stages, and its diagnostic and/or prognostic biomarkers are still insufficient with poor prognosis of patients. To discover new biomarkers, we re-analyzed our mitochondrial proteome database of CCA tissues by using bioinformatics tools for secretory protein nature. Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), was predicted that it was lacking signal peptide and might be secreted into plasma/serum via an unconventional pathway, was selected for the present study.
Methods: Checking expression of CIAPIN1 in tissue and serum of intrahepatic Cholangiocarcinoma (iCCA) patients were analyzed by western blot. CIAPIN1 levels in the pre-operative sera of 46 iCCA patients and 23 healthy controls (HC) were measured using a semi-quantitative dot blot assay. Diagnostic values of serum CIAPIN1 levels were evaluated.
Results: The median level ± quartile deviation of CIAPIN1 was 0.31 ± 0.22 µg/µL and 0.66 ± 0.30 µg/µL in the HC group and iCCA patient group, respectively. CIAPIN1 levels in the sera of iCCA patients were significantly higher than those of healthy controls (p < 0.0001, Mann–Whitney U test). Using a cut-off value of 0.46 µg/µL (at maximal Youden Index), CIAPIN1 provided 71.74% sensitivity, 95.65 % specificity, 79.71 accuracy, and the area under the curve (AUC) was 0.863 (95%CI: 0.779-0.947) for diagnosis of iCCA.
Conclusion: CIAPIN1 might be used as a diagnostic marker for CCA. Furthermore, the expression level of CIAPIN1 in the serum of benign biliary disease patients compare with those of pre-operative serum samples of iCCA patients need to be investigated in the future.
|
Author |
|
Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
Level of Conference |
นานาชาติ |
Type of Proceeding |
Abstract |
Type of Presentation |
Poster |
Part of thesis |
true |
Presentation awarding |
true |
Award Title |
The best poster presentation |
Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
Organiser |
CARI, Faculty of Medicine, KKU |
Date of awarding |
19 ธันวาคม 2563 |
Attach file |
|
Citation |
0
|
|
|
|
|
|
|