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Publication
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| Research Title |
Suppression of sialylation increases sensitivity of glioblastoma cells to cisplatin and 5-fluorouracil |
| Date of Distribution |
6 July 2021 |
| Conference |
| Title of the Conference |
7th BMB conferencce: Challenges in Biochemistry to Overcome Pandomics and Disruotions |
| Organiser |
The Biochemistry an dMolecular Biology Section, The Science Society of Thailand. The Science Society of Thailand Under the Patronage of His Majesty the King |
| Conference Place |
Zoom meeting online |
| Province/State |
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| Conference Date |
6 July 2021 |
| To |
7 July 2021 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
1-6 |
| Editors/edition/publisher |
The Biochemistry and Molecular Biology Section |
| Abstract |
Background and objective: Aberrant sialylation was found to promote tumorigenesis and progres-sion of many cancer types. We aimed to investigate the involvement of sialylation in chemosen-sitivity of glioblastoma cells. Materials and Methods: Expression of sialylated glycans in glio-blastoma cell lines (U373, U251, and U87) was determined by lectin fluorescence using Maackia amurensis lectin II (MAL-II) and Sambucus nigra agglutinin (SNA). The sensitivity of glioblastoma cells to cisplatin and 5-fluorouracfil (5-FU) was investigated by sulforhodamine B assay, after suppression of sialylation by a pan-sialylation inhibitor (3Fax-peracetyl-Neu5Ac, 3Fax). Results: By lectin fluorescence staining, α2,3- and α2,6- sialylated glycans were found to differentially express in U373, U251, and U87 glioblastoma cell lines. Cell viability of U373, U251, and U87 were significantly decreased after combined-treatment of cisplatin or 5-FU with 3Fax, comparing with chemo-drugs or 3Fax alone. This information suggested the involvement of sialylation in chemoresistance of glioblastoma cells. Conclusions: Sialylation was demonstrated in our study to play an important role in chemoresistance of glioblastoma cells, suggesting the potential of us-ing sialylation inhibitor as a chemosensitizer for treatment of glioblastoma in the future. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
false |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย