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Publication
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| Title of Article |
Bioinformatic Prediction of Novel Signaling
Pathways of Apoptosis-inducing Factor,
Mitochondrion-associated 3 (AIFM3) and Their
Roles in Metastasis of Cholangiocarcinoma Cells |
| Date of Acceptance |
24 November 2021 |
| Journal |
| Title of Journal |
Cancer Genomics & Proteomics |
| Standard |
ISI |
| Institute of Journal |
The International Institute of Anticancer Research |
| ISBN/ISSN |
1109-6535 |
| Volume |
2022 |
| Issue |
19 |
| Month |
January |
| Year of Publication |
2022 |
| Page |
35-49 |
| Abstract |
Background/Aim: We previously demonstrated that
a mitochondrial protein, apoptosis-inducing factor,
mitochondrion-associated 3 (AIFM3) is over-expressed in
cholangiocarcinoma (CCA) and its serum levels can be a
prognostic biomarker for CCA. To elucidate the functional
roles of AIFM3 in CCA progression, we aimed to determine
the signaling pathways of AIFM3 in CCA. Materials and
Methods: AIFM3 gene in CCA cells was silenced and AIFM3-
related proteins were identified using mass spectrometry and
bioinformatics tools. The relationships between AIFM3 and
441 related proteins were explored. To validate the functions
of AIFM3, transwell migration/invasion assays were used.
Results: Bioinformatic analyses predicted that AIFM3
interacts with formin-like protein 3 (FMNL3) and is involved
in tumor cell motilities. Online database analysis revealed
higher AIFM3 mRNA expression levels in CCA, particularly
with lymph node metastasis. After AIFM3 gene silencing, CCA
cell migration/invasion was significantly decreased (p<0.001).
Furthermore, AIFM3 expression levels were significantly
associated with lymph node metastasis (p=0.0009) and
shorter survival time (p=0.020). Conclusion: The AIFM3
signaling pathway is mediated via FMNL3 and involved in
metastasis, suggesting that AIFM3 might be a molecular
target to prevent CCA metastasis |
| Keyword |
Cholangiocarcinoma, AIFM3, signaling pathway, bioinformatics, mass spectrometry, metastasis |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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