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Publication
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| Title of Article |
Effects of extenders and cryoprotectants on cryopreservation of Thai red junglefowl (Gallus gallus gallus) spermatozoa |
| Date of Acceptance |
19 April 2022 |
| Journal |
| Title of Journal |
Cryobiology |
| Standard |
SCOPUS |
| Institute of Journal |
Elsevier B.V. Registered Office: Radarweg 29, 1043 NX Amsterdam, The Netherlands, Registered in The Netherlands, Registration No. 33156677, BTW No. NL 005033019B01. |
| ISBN/ISSN |
ISSN: 0011-2240 |
| Volume |
2022 |
| Issue |
106 |
| Month |
1 June 2022 |
| Year of Publication |
2023 |
| Page |
pp. 48-54 |
| Abstract |
Semen cryopreservation is crucial maintain genetic diversity of avian species. Little is known about suitable extenders for post-thaw survival of spermatozoa from Thai red junglefowl (Gallus gallus gallus). Therefore, the present study aimed to compare the suitability of the modified Thai red junglefowl extender (TRJFE) for cryopreservation of Thai red junglefowl spermatozoa with other extenders, including the Schramm extender, the red junglefowl extender (RFE), and the HS1 extender, in terms of sperm viability, motility, and fertility. First, the effects of adding 6% and 9% (v/v) N,N-dimethylacetamide, N,N-dimethylformamide (DMF), or N-methyl acetamide to these extenders on the post-thaw sperm quality of pooled ejaculates from 25 male fowls. The viability of thawed sperm was assessed by using nigrosin–eosin staining and sperm motility was determined by using computer-assisted sperm analysis. Fertility was assessed by inseminating 144 laying hens. The TRJFE + 6% DMF combination significantly improved post-thaw viability (64.88 ± 0.51%) and motility (68.58 ± 1.13%) of Thai red junglefowl sperm, and the semen had the highest fertility (60.97 ± 0.72%). The findings suggest that TRJFE + 6% DMF is a suitable freezing medium to conserve Thai red jungle fowl genetic resources. |
| Keyword |
Cryoprotectant, Extender, Semen cryopreservation, Thai red junglefowl, Fertility |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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