| Title of Article |
The Bth_i0249 Regulates Burkholderia
thailandensis Biofilm Formation |
| Date of Acceptance |
25 May 2022 |
| Journal |
| Title of Journal |
KKU Research Journal (Graduate Studies) |
| Standard |
TCI |
| Institute of Journal |
Graduate School, Khon Kaen University |
| ISBN/ISSN |
|
| Volume |
2023 |
| Issue |
23 |
| Month |
April to June |
| Year of Publication |
2023 |
| Page |
- |
| Abstract |
Biofilm forming bacteria are a structured community of microorganisms enclosed in a self-produced polymatrix. Biofilm forming bacteria are responsible for a variety of chronic infections. Burkholderia pseudomallei is a causative agent of a broad spectrum of clinical symptoms collectively known as melioidosis. Biofilm formation and maturation in Burkholderia subspecies is poorly defined. Burkholderia thailandensis is a closely related subspecies to B. pseudomallei. Because of the broad similarities between these two bacteria, B. thailandensis is sometimes used as a model for study of B. pseudomallei. The aim of this study was to investigate the B. thailandensis biofilms phenotype in static and shear conditions using confocal laser scanning microscopy (CLSM). It was demonstrated that B. thailandensis was able to form mature biofilms over 96 hours that expressed multiple three-dimensional biofilm structures under both static and laminar-shear conditions. Using the microarray data from other Gram-negative bacteria that exhibited differential expression in biofilms as compared with free-living cells it was possible identify a candidate bth_i0249 gene of the YcgR protein superfamily of proteins that contains the PilZ conserved domain of B. thailandensis that plays a role in biofilm formation. Both the Δbth_i0249 and its conserved domain, Δbth_pilZ mutants, significantly reduced biofilm production both in static and laminar-shear conditions. Taken together, the bth_i0249 and its conserved domain, pilZ, are involved in biofilm formation. |
| Keyword |
Biofilm formation, B. thailandensis, Mutation |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
ชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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