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Publication
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Title of Article |
Effect of combination of vitamin-mineral supplementation and concentrateto-roughage ratio diets on ruminal fermentation, gas kinetics and
microbial biomass production using in vitro gas production technique |
Date of Acceptance |
30 August 2021 |
Journal |
Title of Journal |
Agriculture and Natural Resources |
Standard |
SCOPUS |
Institute of Journal |
Kasertsart University Research and Development Institute, Kasertsart University |
ISBN/ISSN |
764-776 |
Volume |
2021 |
Issue |
55 |
Month |
October |
Year of Publication |
2021 |
Page |
13 |
Abstract |
A vitamin-mineral combination consisting of betaine, biotin and chromium picolinate (BBC)
was developed and the effects of BBC supplementation were investigated using an in vitro
gas production technique. The effects were examined on the gas kinetics, microbial biomass
production (MBP) and rumen fermentation. The experimental design was a 3×4 factorial
arrangement in a completely randomized design. Factor A was the concentrate (C)-toroughage (R) ratio of diets with three levels (50C:50R, 60C:40R, 70C:30R) and factor B was
the amount of BBC supplementation with four levels (0 g/kg dry matter (DM); 3 g/kg DM;
6 g/kg DM; 9 g/kg DM). The gas production was recorded at 1 hr, 2 hr, 4 hr, 6 hr, 8 hr, 12
hr, 16 hr, 24 hr and 48 hr of incubation. The gas kinetics, truly digestible organic matter
(TDOM), MBP and efficiency of MBP were measured. Ammonia nitrogen (NH3-N) and total
volatile fatty acids (TVFA) were analyzed in fluid. The results revealed that the gas kinetics
(a and b) did not differ among treatments, but the gas production rate constants (c) increased
when the C:R ratio in diets increased (p < 0.05). TDOM, MBP and EMBP differed (p < 0.05)
among treatments, and the C:R ratios influenced (p < 0.05) the extent of the difference, but
the amount of BBC supplementation did not. The NH3-N concentration was greater (p <0.05)
in diets supplemented with BBC than in a diet without BBC supplementation. Increasing
the C:R ratio in the diet resulted in lower acetic acid and higher propionic acid proportions
(p <0.01). In conclusion, increasing the C:R ratio from 50C:50R to 60C:40R or 70C:30R
resulted in enhanced TDOM, MBP and TVFA. BBC inclusion had no positive effect. |
Keyword |
Betaine, Biotin, Chromium, Gas production, Microbial biomass |
Author |
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Reviewing Status |
มีผู้ประเมินอิสระ |
Status |
ตีพิมพ์แล้ว |
Level of Publication |
นานาชาติ |
citation |
true |
Part of thesis |
true |
Attach file |
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Citation |
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