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Publication
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| Title of Article |
Andrographolide Inhibits Epstein-Barr Virus Lytic Reactiva-tion in EBV-Positive Cancer Cell Lines Through the Modula-tion of Epigenetic-Related Proteins |
| Date of Acceptance |
21 July 2022 |
| Journal |
| Title of Journal |
Molecules |
| Standard |
SCOPUS |
| Institute of Journal |
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
| ISBN/ISSN |
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| Volume |
27 |
| Issue |
14 |
| Month |
๋ีJuly |
| Year of Publication |
2022 |
| Page |
4666 |
| Abstract |
Reactivation of Epstein-Barr virus (EBV) is associated with EBV-associated malignancies and considered to be a benefit target for treatment. Andrographolide is claimed to have antiviral and anti-tumor activities. Therefore, this study aimed to investigate the effect of andrographolide on the inhibition of EBV lytic reactivation in EBV-positive cancer cells. The cytotoxicity of andro-grapholide was firstly evaluated in EBV-positive cancer cells; P3HR1, AGS-EBV and HONE1-EBV cells, using MTT assay. Herein, the spontaneous expression of EBV lytic genes; BALF5, BRLF1 and BZLF1, was significantly inhibited in andrographolide-treated cells. Accordingly, andro-grapholide inhibited the expression of Zta and viral production in sodium butyrate (NaB)-induced EBV lytic reactivation. Additionally, proteomics and bioinformatics analysis revealed the differentially expressed proteins that inhibit EBV lytic reactivation in all treated cell lines were functionally related with the histone modifications and chromatin organization, such as histone H3-K9 modification and histone H3-K27 methylation. Taken together, andrographolide inhibits EBV reactivation in EBV-positive cancer cells by inhibiting EBV lytic genes, probably, through the histone modifications. |
| Keyword |
andrographolide; Epstein-Barr virus; lytic reactivation; BZLF1; histone modifications |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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