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Publication
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| Title of Article |
Characterization and Involvement of Exosomes Originating from Chikungunya Virus-infected Epithelial Cells in the Transmission of Infectious Viral Elements |
| Date of Acceptance |
8 October 2022 |
| Journal |
| Title of Journal |
International Journal of Molecular Sciences |
| Standard |
OTHER (SCIE) |
| Institute of Journal |
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the termsand conditions of the Creative Commons Attribution (CC BY) license |
| ISBN/ISSN |
1661-6596 |
| Volume |
2022 |
| Issue |
23 |
| Month |
October |
| Year of Publication |
2022 |
| Page |
12117 |
| Abstract |
The Chikungunya virus (CHIKV) is a mosquito‐borne alphavirus that affects the world’s
population with chikungunya disease. Adaptation of the viral life cycle to their host cells’ environment
is a key step for establishing their infection and pathogenesis. Recently, the accumulating evidence
advocates a principal role of extracellular vesicles (EVs), including exosomes, in both the
infection and pathogenesis of infectious diseases. However, the participation of exosomes in CHIKV
infection and transmission is not well clarified. Here, we demonstrated that the CHIKV RNA and
proteins were captured in exosomes, which were released by viral‐infected epithelial cells. A viral
genomic element in the isolated exosomes was infectious to naïve mammalian epithelial cells. The
assay of particle size distribution and transmission electron microscopy (TEM) revealed CHIKVderived
exosomes with a size range from 50 to 250 nm. Treatments with RNase A, Triton X‐100, and
immunoglobulin G antibodies from CHIKV‐positive patient plasma indicated that infectious viral
elements are encompassed inside the exosomes. Interestingly, our viral plaque formation also exhibited
that infectious viral elements might be securely transmitted to neighboring cells by a secreted
exosomal pathway. Taken together, our recent findings emphasize the evidence for a complementary
means of CHIKV infection and suggest the role of exosome‐mediated CHIKV transmission. |
| Keyword |
chikungunya virus (CHIKV); extracellular vesicles; EVs; exosomes; cell‐to‐cell transmission; infectious viral elements |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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