บทคัดย่อ |
The effectiveness of column bioreactors for butanol fermentation from sugarcane molasses
by Clostridium beijerinckii TISTR 1461 was investigated. This fermentation was operated at
an initial pH of 6.5 and temperature of 37 C under anaerobic conditions. A 1-L bubble column
bioreactor was used with various gas circulation rates ranging from 0.2 to 1.0 L/min. The highest
butanol concentration (PB, 8.72 g/L), productivity (QB, 0.24 g/Lh) and yield (YB/S, 0.21 g/g) were
obtained with a gas circulation of 0.2 L/min. To improve butanol production efficiency, gas-lift
column bioreactors with internal and external loops at 0.2 L/min of circulating gas were used. Higher
PB (10.50–10.58 g/L), QB (0.29 g/Lh) and YB/S (0.22–0.23 g/g) values were obtained in gas-lift column
bioreactors. These values were similar to those using a more complex 2-L stirred-tank bioreactor
(PB, 10.10 g/L; QB, 0.28 g/L h and YB/S, 0.22 g/g). Hence, gas-lift column bioreactors have potential
for use as low-cost fermenters instead of stirred-tank bioreactors for butanol fermentation. When the
gas-lift column bioreactor with an internal loop was coupled with a gas stripping system, it yielded
an enhanced PB and sugar consumption of approximately 9% and 7%, respectively, compared to a
system with no gas stripping. |