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Publication
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| Title of Article |
Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress |
| Date of Acceptance |
24 May 2023 |
| Journal |
| Title of Journal |
PLos ONE |
| Standard |
SCOPUS |
| Institute of Journal |
PLOS ONE |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2023 |
| Page |
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| Abstract |
Helianthus tuberosus L. (Jerusalem artichoke) produce inulin, a type of fructan, which
possesses several biotechnology applications, e.g., sugar syrup, prebiotics, fiber in diabetic food,
enabling blood sugar and cholesterol reduction. Drought reduces inulin accumulation in the
tubers of Jerusalem artichoke as the plants protect themselves from this stress by induction of
stress gene responses, effecting growth reduction. Endophytic bacteria are promising candidates
to promote plant growth and yield particularly under abiotic stress. Therefore, three endophytic
bacteria with plant growth promoting properties were examined for their ability to improve
Jerusalem artichoke growth and yield under both well-watered and drought conditions when
inoculated individually or in combinations in pot experiments with 2 factorial random complete
block design. The interactions of the endophytic bacteria and plant host determined the
differential gene expression in response to drought as revealed by quantitative polymerase chain
reaction. Single inoculum of the endophytic bacteria increased the height, weight, root traits, and
harvest index of Jerusalem artichoke compared to co-inocula under both well-watered and
drought conditions. However, the co-inocula of Rossellomorea aquimaris strain 3.13 and
Bacillus velezensis strain 5.18 proved to be a synergistic combination leading to high inulin
accumulation; while the co-inocula of B. velezensis strain 5.18 and Micrococcus luteus strain
4.43 were not beneficial when used in combination. The genes, dehydrin like protein and
ethylene responsive element binding factor, were upregulated in the plants inoculated with single
inoculum and co-inocula of all endophytic bacteria during drought stress. Moreover, the gene
expression of indole-3-acetic acid (IAA) amido synthetase were up-regulated in Jerusalem
artichoke inoculated with M. luteus strain 4.43 during drought stress. The fructan:fructan 1-
fructosyltransferase (1-FFT) was also stimulated by the endophytic bacteria particularly in
3
drought condition; the results of this study could explain the relationship between endophytic
bacteria and plant host for growth and yield promotion under well-watered and drought
conditions. |
| Keyword |
ethylene response, inulinase, inulin synthesis, phytohormone, 1-FFT |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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