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Publication
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| Title of Article |
Slight changes of gut microbiome in the early stages of chronic kidney disease of unknown etiology |
| Date of Acceptance |
23 June 2023 |
| Journal |
| Title of Journal |
Microbes and Environments |
| Standard |
SCOPUS |
| Institute of Journal |
Japanese Society Of Microbial Ecology |
| ISBN/ISSN |
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| Year of Publication |
2023 |
| Page |
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| Abstract |
Gut dysbiosis and alteration of short-chain fatty acids (SCFAs) are seen in end-stage chronic kidney disease (CKD). However, the degree of change of the gut microbiome and serum SCFA profiles in the early stages of CKD, particularly in CKD of unknown etiology (CKDu), is unclear. We aimed to investigate the gut microbiome and SCFA profiles in early-stage CKD patients (CKD stages 1-3) in a community at Khon Kaen Province, Thailand. Seventy-two parasite-free participants were distributed among a healthy group (HC, n=18) and three patient groups (an underlying disease group (UD, n=18), early-stage CKD with underlying disease (CKD-UD, n=18) and early-stage CKD of unknown etiology, CKDu, n=18). Fecal DNA was individually extracted and pooled for groups of six individuals (three pools/group) to study gut microbiome composition using next-generation sequencing. SCFA analysis was performed on a serum sample from each individual using gas chromatography-mass spectrometry. Results revealed that microbial abundance differed between the healthy group and all patient groups (UD, CKD-UD and CKDu). The [Eubacterium]_coprostanoligenes_group was enriched in the CKDu group compared to HC and CKD-UD groups. In addition, acetate, a major SCFA component, was also significantly reduced in the serum of all patient groups compared to the HC group. Our results indicate that minor changes of gut microbiome and a significant decrease in serum acetate occur in early-stage CKDu, which might be important information for developing prevention strategies in CKD patients. |
| Keyword |
Gut microbiome, chronic kidney disease, 16S rRNA, Next-generation sequencing, short-chain fatty acids |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย