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ข้อมูลการเผยแพร่ผลงาน
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| ชื่อบทความ |
Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts |
| วัน/เดือน/ปี ที่ได้ตอบรับ |
15 สิงหาคม 2566 |
| วารสาร |
| ชื่อวารสาร |
Open Veterinary Journal |
| มาตรฐานของวารสาร |
SCOPUS |
| หน่วยงานเจ้าของวารสาร |
Faculty of Veterinary Medicine, University of Tripoli, Libya |
| ISBN/ISSN |
E-ISSN 2218-6050 | ISSN 2226-4485 |
| ปีที่ |
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| ฉบับที่ |
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| เดือน |
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| ปี พ.ศ. ที่พิมพ์ |
2566 |
| หน้า |
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| บทคัดย่อ |
Background: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced.
Aim: This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of CCA from susceptible and non-susceptible animal models.
Methods: Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and non-susceptible animal models, were used in this comparative study. The animals were infected with 50 O. viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 post-infection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal-Wallis and Mann-Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value <0.05.
Results: The results demonstrated that mice had different patterns of infiltrating cell types when compared to hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56. Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p=0.047), D7 (p=0.049), D28 (p=0.040), and D56 (p<0.040).
Conclusion: The inflammatory responses to O. viverrini infection in the non-susceptible animal model occurred and declined earlier while the response in the susceptible animal model occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis susceptibility.
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| คำสำคัญ |
inflammatory cells, biliary, Opisthorchis viverrini, susceptible host, non-susceptible host |
| ผู้เขียน |
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| การประเมินบทความ |
มีผู้ประเมินอิสระ |
| สถานภาพการเผยแพร่ |
ได้รับการตอบรับให้ตีพิมพ์ |
| วารสารมีการเผยแพร่ในระดับ |
นานาชาติ |
| citation |
มี |
| เป็นส่วนหนึ่งของวิทยานิพนธ์ |
เป็น |
| แนบไฟล์ |
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| Citation |
0
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