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Publication
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| Research Title |
Evaluation the Anticancer Effect of Sulfated Galactans and
Its Modifications on Human Breast Cancer MCF-7 Cell Line |
| Date of Distribution |
3 May 2023 |
| Conference |
| Title of the Conference |
The 45th Annual Conference of the Anatomy Association of Thailand |
| Organiser |
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Anatomy Association of Thailand (AAT) |
| Conference Place |
Suan Bua Hotel & Resort, Chiang Mai |
| Province/State |
Chiang Mai, Thailand |
| Conference Date |
3 May 2023 |
| To |
5 May 2013 |
| Proceeding Paper |
| Volume |
2023 |
| Issue |
- |
| Page |
18 |
| Editors/edition/publisher |
- |
| Abstract |
Natural sulfated polysaccharides (SP) from seaweed have been shown to inhibit cell cycle progression in several cancers. Sulfated galactans (SG), a SP from the red seaweed Gracilaria fisheri, had an inhibitory effect on cholangiocarcinoma cell proliferation through cell cycle arrest. Recently, it has been demonstrated that the chemical structure modification of SP can enhance
its bioactive properties. Nevertheless, the effect of SG and its modifications on breast cancer has not been explored. Therefore, this study was focused on the anticancer effect of SG and its modifications on human breast cancer MCF-7 cell line and the evaluation of cell cycle regulatory proteins. SG isolated from G. fisheri was modified to obtain the degraded-SG (DSG) and
lauroyl-SG (LSG). The SG, DSG, and LSG were then characterized by NMR and FTIR. MCF-7 cells were cultured and treated with the different concentrations of SG, DSG, and LSG for 24 and 48 h. MCF-7 Cell proliferation was investigated using MTT and immunofluorescent staining assays. After treatment expression of cell cycle regulatory proteins of MCF-7 was determined by Western blotting assay. NMR and FTIR results showed that SG was successfully modified. SG, DSG, and LSG tended to suppress MCF-7 cell proliferation expressing the ability to inhibit the
expression of cyclin and cyclin-dependent kinase cell cycle regulatory proteins. These findings implicated that SG, and its modifications may have significant potential as therapeutic agents for breast cancer. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย