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Publication
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| Title of Article |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis. |
| Date of Acceptance |
16 December 2009 |
| Journal |
| Title of Journal |
Veterinary Parasitology |
| Standard |
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| Institute of Journal |
Elsevier Science |
| ISBN/ISSN |
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| Volume |
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| Issue |
168 |
| Month |
มีนาคม |
| Year of Publication |
2010 |
| Page |
255-260 |
| Abstract |
A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with
melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito
vectors and dog blood samples was developed. This real-time FRET PCR was based on the
fluorescence melting curve analysis of a hybrid between an amplicon generated from the
D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The
sensitivity, specificity, accuracy, and positive and negative predictive values of this
method were all 100%. Besides being highly sensitive and specific, this PCR is fast and
offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and
for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring
of D. immitis in mosquito vectors. |
| Keyword |
Dirofilaria immitis, Detection, Dogs, Mosquito, Parasite, Real-time PCR |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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