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Publication
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| Title of Article |
Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| Date of Acceptance |
8 December 2007 |
| Journal |
| Title of Journal |
The American Journal of Tropical Medicine and Hygiene |
| Standard |
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| Institute of Journal |
Allen Press |
| ISBN/ISSN |
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| Volume |
78 |
| Issue |
3 |
| Month |
มกราคม |
| Year of Publication |
2010 |
| Page |
509-513 |
| Abstract |
We developed real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR)
combined with melting curve analysis for detection of Brugia malayi DNA in blood-fed mosquitoes. Real-time FRET
PCR is based on a fluorescence melting curve analysis of hybrid formed between amplicons generated from a family of
repeated DNA element, 153-bp HhaI repeated sequence, specific to genus Brugia and specific fluorophore-labeled
probes. The B. malayi–infected mosquitoes were differentiated from Wuchereria bancrofti–infected and uninfected
mosquitoes and from genomic DNA of Dirofilaria immitis– and Plasmodium falciparum–infected human red blood cells
and human leukocytes by their melting temperature. Sensitivity and specificity were both 100%. Melting curve analysis
produces a rapid, accurate, and sensitive alternative for specific detection of B. malayi in mosquitoes, allows high
throughput, and can be performed on small samples. This method has the potential for endemic area mapping or
monitoring effect of brugian filariasis mass treatment programs. |
| Keyword |
Brugia malayi , Real-time PCR, Mosquito, Detection |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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