| Research Title |
Development of multiplex PCR for detection of superantigenic toxin genes in Staphylococcus aureus isolates in Thailand |
| Date of Distribution |
10 September 2011 |
| Conference |
| Title of the Conference |
XIII International Congress of Bacteriology and Applied Microbiology |
| Organiser |
International Union of Microbiological Societies (IUMS) 2011 |
| Conference Place |
Sapporo Convention Center, Sapporo Business Innovation Center |
| Province/State |
Shiroishi-ku, Sapporo, Japan |
| Conference Date |
6 September 2011 |
| To |
10 September 2011 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
Fusao Tomita |
| Abstract |
Superantigenic toxins produced by Staphylococcus aureus are one of the most important virulent factors of food poisoning, septicemia as well as toxic shock syndrome. Objectives: To develop multiplex PCR for detection of superantigenic toxin genes (sea, seb, sec, sed and tst-1) in methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus (MSSA) isolated from clinical samples (blood, sputum, pus and urine) in patients and healthy nasal carriers, and to investigate the correlation between toxin genes and toxin production. Methods: Total of 186 S. aureus isolates (149 isolates from clinical samples and 37 isolates from nasal carriers) were investigated toxin-harbored genes by multiplex PCR. The methicillin resistance was determined by carrying mecA gene and/or resistance to cefoxitin disc (30 µg). The toxin production was determined by reverse passive latex agglutination (RPLA). Results: The sensitivity of multiplex PCR for simultaneous detection of five toxin genes was 22 cfu and as low as 2-16 cfu for the uniplex PCR. The superantigenic toxin genes were detected in 62.4% (116/186). 96/149 (64.6%) of clinical S. aureus isolates and 20/37 (54.1%) of nasal carriers possessed superantigenic toxin genes, respectively. The individual of sea gene showed the highest incidence in all of specimens except from urine samples but there was no significant association between toxin genotype and specimen type. In addition, MRSA showed more statistically significant in harboring sea gene than MSSA (P<0.05). The detection of superantigenic toxin genes corresponded with RPLA method in 77.8%. Conclusions: The multiplex PCR can be applied for simultaneous detection of the virulence genes in S. aureus isolated from clinical sources and can be used for surveillance of S. aureus virulence strains spreading in the hospital and environment. |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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