2012 ©
Journal Publication
Title of Article Ovarian responses and FSH profiles at superovulation with a single epidural administration of gonadotropin in the Thai-Holstein crossbreed 
Date of Acceptance 6 October 2021 
     Title of Journal Animal Reproduction (AR) 
     Standard ISI 
     Institute of Journal Brazilian College of Animal Reproduction  
     Year of Publication 2021 
     Abstract The conventional method of ovarian superstimulation requires multiple injections of gonadotropins which is time-consuming and may be stressful for the cows. This study was designed to determine whether a single epidural injection of FSH (EI group) would induce the superovulatory response in the Thai-Holstein crossbreed and evaluate FSH plasma hormone concentrations. Eight cows (replication = 3; n=24) were assigned to one of 2 treatments in switch back design. Control group (n=12): cows were received 400 mg FSH twice daily by intramuscularly for 4 days (80, 80, 60, 60, 40, 40, 20 and 20 mg), EI group (n=12): cows were received 400 mg FSH by single epidural injection. Data were collected in term of ovarian follicle responses, superovulatory responses, ova/embryo collection. FSH concentrations were examined using ELISA. The total follicular responses during oestrus were not different between treatments; however, the large follicles were less frequent (P<0.01) while the medium follicle sizes were higher (P<0.05) in the EI group. The plasma concentration of FSH in EI was dramatically increased within 2 hours before decreasing sharply thereafter (P<0.01) and did not remain above baseline after 10 h of administration. The embryo quality was better in the control than the EI groups (P<0.05). Interestingly, the number of ovulation cysts in the EI group was 50%. The ovarian responses and embryo quality in the cows with cysts were worse compared with the non-cyst groups (P<0.05). In conclusion, alternative protocols decreased the superovulatory response and increased poor embryo quality in Thai-Holstein crossbred. Also, the incidence of ovarian follicular cysts is higher in the EI group. 
     Keyword Embryo transfer, superstimulation, dairy cows, ovarian cysts 
615030039-6 Miss RUJIRA CHUMCHAI [Main Author]
Agriculture Master's Degree
605030022-2 Miss THANAPORN RATSIRI
Agriculture Master's Degree
Agriculture Doctoral Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ได้รับการตอบรับให้ตีพิมพ์ 
Level of Publication นานาชาติ 
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