| Title of Article |
Characterization of exo-inulinase from endophytic Bacillus aquimaris 3.13 for digestion
of inulin in Jerusalem artichoke tubers |
| Date of Acceptance |
8 July 2018 |
| Journal |
| Title of Journal |
Food Science and Biotechnology |
| Standard |
SCOPUS |
| Institute of Journal |
Springer Journals Editorial Office |
| ISBN/ISSN |
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| Volume |
|
| Issue |
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| Month |
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| Year of Publication |
2018 |
| Page |
21 |
| Abstract |
Inulinase is widely used in the food industry as it can digest substrates from various
sources and is effective in producing high yield product. Purification and
characterization of inulinase took place on samples from an endophytic bacterium,
Bacillus aquimaris 3.13 isolated from Jerusalem artichoke. The purified enzyme
revealed molecular mass of 58 kDa assessed by SDS-PAGE. The optimum
temperature and pH were 60°C and pH 5.0, respectively. Enzyme activity was stable
up to 80°C for 30 min, remaining 80% activity and also stable in a pH range of 5-7 for
12 hours. This inulinase had a substrate specific to levan; moreover, it also digested
inulin and raffinose. Thin layer chromatography analysis of the end product revealed
that inulinase hydrolyzed inulin, giving only fructose; therefore, the purified enzyme is
an exo-inulinase. This study indicated that inulinase from B. aquimaris 3.13 can be
used in fructose production from inulin and levan substrates. |
| Keyword |
exo- inulinase; fructan; fructose syrup; inulin; levan |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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