| Title of Article |
Efficient precipitation methods of inulinase from endophytic bacteria bacillus aquimaris isolated from jerusalem artichoke |
| Date of Acceptance |
22 November 2018 |
| Journal |
| Title of Journal |
International Journal of Applied and Physical Sciences (IJAPS) |
| Standard |
|
| Institute of Journal |
KKG publication |
| ISBN/ISSN |
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| Volume |
4 |
| Issue |
3 |
| Month |
|
| Year of Publication |
2018 |
| Page |
|
| Abstract |
Inulinase is an enzyme that hydrolyzes inulin to oligosaccharide used in the food industry. The endophytic bacteria, Bacillus aquimaris3.13 isolated from jerusalem artichoke, obtained inulinase when grown in luriabertani medium (LB broth) containing 1% inulin as the carbon and energy source with incubation temperature at 37°C for 20 hours and shaking speed of 150 rpm.
The sample showed inulinase activities at 729 U after incubation at 55 °C for 20 min in the presence of inulin substrate. There were four methods for precipitation : ammonium sulfate, ethanol, butanol, and 2-steps of salt and alcohol. The best precipitated protein method was 50% of ethanol, giving 53% protein recovery (388 U activity). |
| Keyword |
Inulin ,Inulinase ,Endophytic bacteria |
| Author |
|
| Reviewing Status |
ไม่มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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